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Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering

Received: 23 February 2018     Accepted: 9 March 2018     Published: 2 April 2018
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Abstract

In this research work, gelatin-carboxymethylchitosan (CMC) based biodegradable composites films were prepared by solution casting method. Chitosan from waste prawn shell was the basic raw materials of CMC synthesis. Five sets of CMC-gelatin composites (5-25 wt% CMC) along-with pure gelatin were prepared in solution casting method. Incorporation of CMC into gelatin significantly altered some of the properties. The CMC and gelatin-CMC composites formation was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). Surface morphology of the films was investigated by Scanning Electron Microscopy (SEM) and SEM micrograph revealed that composites were porous and CMC was homogenously dispersed into gelatin. The porous surface of the composites is one of the criterions for new cells growth. Thermal stability of composites were investigated by thermogravimetric analysis (TGA) and composites more thermal stable (less weight loss) than pure gelatin. Antimicrobial and cytotoxicity tests found all composites were performed microbial safe and no cytotoxic effect. The physico-chemical analyses and others analyses of scaffolds revealed for their application as a wound dressing material or artificial skin.

Published in International Journal of Materials Science and Applications (Volume 7, Issue 2)
DOI 10.11648/j.ijmsa.20180702.15
Page(s) 62-68
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Carboxymethyl Chitosan, Gelatin, Scaffold, Cytotoxicity and Tissue Engineering

References
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[4] Chapekar, M. S., Tissue engineering: challenges and opportunities. Journal of Biomedical Materials Research Part A, 2000. 53(6): p. 617-620.
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[7] Baek, J., et al., Meniscal tissue engineering using aligned collagen fibrous scaffolds: comparison of different human cell sources. Tissue Engineering Part A, 2018. 24(1-2): p. 81-93.
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[12] Wang, G., et al., Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats. Biotechnology Letters, 2010. 32(1): p. 59.
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[16] Chen, X.-G. and H.-J. Park, Chemical characteristics of O-carboxymethyl chitosans related to the preparation conditions. Carbohydrate Polymers, 2003. 53(4): p. 355-359.
[17] Thi, T. T. H., et al., Oxidized cyclodextrin-functionalized injectable gelatin hydrogels as a new platform for tissue-adhesive hydrophobic drug delivery. RSC Advances, 2017. 7(54): p. 34053-34062.
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  • APA Style

    Nisat Tamanna Nipu, Farzana Khan Rony, Asaduz Zaman. (2018). Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering. International Journal of Materials Science and Applications, 7(2), 62-68. https://doi.org/10.11648/j.ijmsa.20180702.15

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    ACS Style

    Nisat Tamanna Nipu; Farzana Khan Rony; Asaduz Zaman. Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering. Int. J. Mater. Sci. Appl. 2018, 7(2), 62-68. doi: 10.11648/j.ijmsa.20180702.15

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    AMA Style

    Nisat Tamanna Nipu, Farzana Khan Rony, Asaduz Zaman. Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering. Int J Mater Sci Appl. 2018;7(2):62-68. doi: 10.11648/j.ijmsa.20180702.15

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  • @article{10.11648/j.ijmsa.20180702.15,
      author = {Nisat Tamanna Nipu and Farzana Khan Rony and Asaduz Zaman},
      title = {Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering},
      journal = {International Journal of Materials Science and Applications},
      volume = {7},
      number = {2},
      pages = {62-68},
      doi = {10.11648/j.ijmsa.20180702.15},
      url = {https://doi.org/10.11648/j.ijmsa.20180702.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmsa.20180702.15},
      abstract = {In this research work, gelatin-carboxymethylchitosan (CMC) based biodegradable composites films were prepared by solution casting method. Chitosan from waste prawn shell was the basic raw materials of CMC synthesis. Five sets of CMC-gelatin composites (5-25 wt% CMC) along-with pure gelatin were prepared in solution casting method. Incorporation of CMC into gelatin significantly altered some of the properties. The CMC and gelatin-CMC composites formation was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). Surface morphology of the films was investigated by Scanning Electron Microscopy (SEM) and SEM micrograph revealed that composites were porous and CMC was homogenously dispersed into gelatin. The porous surface of the composites is one of the criterions for new cells growth. Thermal stability of composites were investigated by thermogravimetric analysis (TGA) and composites more thermal stable (less weight loss) than pure gelatin. Antimicrobial and cytotoxicity tests found all composites were performed microbial safe and no cytotoxic effect. The physico-chemical analyses and others analyses of scaffolds revealed for their application as a wound dressing material or artificial skin.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering
    AU  - Nisat Tamanna Nipu
    AU  - Farzana Khan Rony
    AU  - Asaduz Zaman
    Y1  - 2018/04/02
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijmsa.20180702.15
    DO  - 10.11648/j.ijmsa.20180702.15
    T2  - International Journal of Materials Science and Applications
    JF  - International Journal of Materials Science and Applications
    JO  - International Journal of Materials Science and Applications
    SP  - 62
    EP  - 68
    PB  - Science Publishing Group
    SN  - 2327-2643
    UR  - https://doi.org/10.11648/j.ijmsa.20180702.15
    AB  - In this research work, gelatin-carboxymethylchitosan (CMC) based biodegradable composites films were prepared by solution casting method. Chitosan from waste prawn shell was the basic raw materials of CMC synthesis. Five sets of CMC-gelatin composites (5-25 wt% CMC) along-with pure gelatin were prepared in solution casting method. Incorporation of CMC into gelatin significantly altered some of the properties. The CMC and gelatin-CMC composites formation was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). Surface morphology of the films was investigated by Scanning Electron Microscopy (SEM) and SEM micrograph revealed that composites were porous and CMC was homogenously dispersed into gelatin. The porous surface of the composites is one of the criterions for new cells growth. Thermal stability of composites were investigated by thermogravimetric analysis (TGA) and composites more thermal stable (less weight loss) than pure gelatin. Antimicrobial and cytotoxicity tests found all composites were performed microbial safe and no cytotoxic effect. The physico-chemical analyses and others analyses of scaffolds revealed for their application as a wound dressing material or artificial skin.
    VL  - 7
    IS  - 2
    ER  - 

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Author Information
  • Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka, Bangladesh

  • Institute of Glass and Ceramic Research and Testing, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh

  • Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka, Bangladesh

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